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Wednesday, November 11, 2020 | History

2 edition of Lipid metabolism in tissue culture cells. found in the catalog.

Lipid metabolism in tissue culture cells.

Lipid metabolism in tissue culture cells.

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Published by Wistar Institute Press in Philadelphia .
Written in English

    Subjects:
  • Lipids -- Metabolism -- Congresses.

  • Edition Notes

    StatementEdited by George H. Rothblat [and] David Kritchevsky. Editorial assistant: Pamela W. Brown.
    SeriesThe Wisstar Institute symposium monograph, no. 6
    ContributionsRothblat, George H., ed., Kritchevsky, David, 1920- ed., Wistar Institute of Anatomy and Biology.
    Classifications
    LC ClassificationsQP751 .L55 1966
    The Physical Object
    Paginationvii, 164 p.
    Number of Pages164
    ID Numbers
    Open LibraryOL5549168M
    LC Control Number67028150

      Project Methods This project will utilize in vivo and in vitro cow models with differing steroidogenic capacity within the large and small cells of the CL. Proteomic, lipidomic, and metabolomic profiling will provide the first complete analysis of the protein and lipid compositions of lipid droplets and metabolic regulation of bovine small and large luteal cells. Metabolism (/ m ə ˈ t æ b ə l ɪ z ə m /, from Greek: μεταβολή metabolē, "change") is the set of life-sustaining chemical reactions in three main purposes of metabolism are: the conversion of food to energy to run cellular processes; the conversion of food/fuel to building blocks for proteins, lipids, nucleic acids, and some carbohydrates; and the elimination of. A comprehensive, fun and entertaining site devoted exclusively to histology. Learning histology was never so easy! This site includes histology quizzes, histology games, slides, mnemonics, histology puzzles and tons of information about histology. One of .


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Lipid metabolism in tissue culture cells. Download PDF EPUB FB2

Lipid metabolism in tissue culture cells. Philadelphia, Wistar Institute Press, (OCoLC) Online version: Lipid metabolism in tissue culture cells. Philadelphia, Wistar Institute Press, (OCoLC) Material Type: Conference publication: Document Type: Book: All Authors / Contributors.

LIPID METABOLISM OF ANIMAL CELLS IN CULTURE - A REVIEW Georg Schmid Central Research Laboratories, ZFE/MB, Bid. 66/, F. Hoffmann-La Roche AG, CH Basle, Switzerland ABSTRACT The fatty acid composition of mammalian cells with respect to cellular storage lipids and membrane phospholipids reflects that of lipids present in the culture by: 7.

Lipid metabolism in mammalian tissues and its control by retinoic acid skeletal muscle and white adipose tissue in the context of lipid metabolism is depicted. expression in hepatic cells. Lipid Metabolism in Cultured Cells XI. UTILIZATION OF SERUM TRIGLYCERIDES* by cells in tissue culture to determine its role as a source of lipid When conversion of exogenous triglyceride to other cellular for cells grown in the presence of serum, and to study the mecha- lipids was followed, or when depletion of individual lipid con.

Abstract. Lipid droplets have long been recognized by light microscopy to accumulate in hypoxic cells both in vivo and in the present tissue culture experiments, correlative electron microscopic observations and lipid analyses were performed to determine the nature and significance of lipid accumulation in by: Publisher Summary.

This chapter presents the adipose tissue metabolism of white adipose tissue that is derived from the studies in vivo and in ement of the rates of fatty acid synthesis (FAS) and fatty acid esterification using 3 H 2 O in vivo requires minimal stress to the animal.

Therefore, it is important to accustom animals to handling and to their surroundings because. Lipid metabolism of developing central nervous tissues in organotypic cultures. A comparative study of medium and tissue fatty acids in developing rat cerebral cortex and cerebellum.

Nutr. Metabol. – Google ScholarCited by: Further, cells almost reach confluence at 48 hrs of lipid accumulating and thus, several aspects of lipid metabolism may have been altered [18,19].

At neither 24 nor 48 hrs did ACTH influenced the cellular lipid pool or lipids secreted in HepG2 cells. Nor was any affect of ACTH on lipid output seen at earlier time points (4 or 16 hrs).Cited by: 2.

Adipose Tissue and Lipid Metabolism. In book: Sturkie's Avian Physiology, pp Chicken ES cells were differentiated into osteoblasts. The lipid-desaturation signature was identified in laboratory cultures of ovarian cancer stem cells and in cells from human patients. Higher lipid unsaturation levels also were detected in cancer stem cell-enriched laboratory cultures called “spheroids,” a three-dimensional culture that mimics tumors in human patients.

Abstract. Tissue explants from frontal lobes of rat brain were used for the study of cerebral fatty acid metabolism. After tissues had been maintained in serum-supplemented medium, a lipid free medium was substituted and metabolic studies were carried by:   Growth, Nutrition, and Metabolism of Cells in Culture, Volume 3, focuses on a number of specific, timely areas of research that make use of cell and tissue culture.

The major theme of this volume is growth and its regulation in animal Edition: 1. This Special Issue is devoted to examining lipid and lipoprotein metabolism, the functions of their metabolites in different organisms (using tissue culture and in vivo models), as well as the identification and characterization of novel metabolites.

Robert J. Brown Guest Editor. Manuscript Submission Information. bio chemistry microbiology Books. Molecular Biology Methods and Tissue Culture Methods.

Author(s): University of Maryland, Baltimore County. NA Pages. Nutrition, Amino Acid Metabolism, Bioenergetics, Carbohydrate Metabolism, Lipid Metabolism, Purine and Pyrimidine Metabolism, Bioenergetics, Enzymology, Heme Metabolism, Lipid Metabolism.

Metabolic disease - Metabolic disease - Disorders of lipid metabolism: Lipids are large, water-insoluble molecules that have a variety of biological functions, including storing energy and serving as components of cellular membranes and lipoproteins.

Cells that line the small intestine absorb dietary lipids and process them into lipoprotein particles that enter the circulation via. Anchored-lipid raft AKT protein has been extensively investigated in cancer cells. 62, 63 Its aberrant activation, contributing to tumor development and invasiveness, 64, 65 correlates with Cited by:   The Role of Lipid Metabolism for Neural Stem Cell Regulation Issue title: New Players in Keywords: Neurogenesis, neural stem cells, lipid metabolism, lipogenesis, fatty acid oxidation, essential fatty acids, dietary lipids,again pointing towards a need for modifying current culture by:   FABP4 and FABP5 are important for the maintenance, longevity and function of CD8+ tissue-resident memory T cells, which use oxidative metabolism of exogenous free fatty acids to persist in tissues Cited by: Adipose tissue and lipid metabolism David A.

Bernlohr, Anne E. Jenkins and Assumpta A. Bennaars up approximately one-half of the cells in adipose tissue, the remainder being blood and endothelial cells, adipose precursor cells of varying degrees of differentiation, and used to induce terminal differentiation in culture [1].

Following File Size: 2MB. A triglyceride molecule (a) breaks down into a monoglyceride (b). Lipid metabolism begins in the intestine where ingested triglycerides are broken down into smaller chain fatty acids and subsequently into monoglyceride molecules (see b) by pancreatic lipases, enzymes that break down fats after they are emulsified by bile food reaches the small intestine in the.

In mammals, white adipose tissue functions as the main depot for fuel storage. In the past decade, identification of myriad lipid and protein signals secreted from this tissue has led to its recognition as a major endocrine organ (Rondinone, ; Trayhurn and Bing, ).This was principally based on the discovery of leptin, a cytokine-like hormone secreted from white.

This chapter focuses on the biochemistry of lipid metabolism in the adipocyte. Adipocytes make up approximately one-half of the cells in adipose tissue, the remainder being blood and endothelial cells, adipose precursor cells of varying degrees of Cited by: 4.

Transport substances that can't pass because: too large, not lipid-soluble, going against concentration gradient. 2 forms, solute pumping, bulk transport. Solute Pumping Amino acids, some sugars, and ions are transported by solute pumps.

Peng et al. find that Cyp2r1 depletion results in 1,25(OH)2D3 deficiency, retarded growth, and excessive visceral adipose tissue (VAT) in zebrafish. 1,25(OH)2D3 regulates lipid metabolism through the regulation of Pgc1a, controlling mitochondrial biogenesis and oxidative metabolism in Cited by: Chemically defined lipid concentrate is a concentrated lipid emulsion designed to reduce or replace fetal bovine serum in cell culture media for a wide variety of applications, including growth and maintenance of CHO, hybridoma, and insect cells in culture; monoclonal antibody production by hybridomas; and viral expression in insect cells.

Past and present. As early as inMann and Spoerry observed that inhabitants from African Maasai tribes maintained a lower level of blood lipids due to a high fermented milk intake [].Further perspective suspected that live Lactobacilli included in fermented milk may contribute to reducing cholesterol [].The cholesterol-reducing effect of probiotic has become more apparent Cited by: 3.

Lipid, any of a diverse group of organic compounds including fats, oils, hormones, and certain components of membranes that are grouped together because they do not interact appreciably with type of lipid, the triglycerides, is sequestered as fat in adipose cells, which serve as the energy-storage depot for organisms and also provide thermal insulation.

Specific metabolic processes regulated by adipose tissue include lipid metabolism, glucose homeostasis, inflammation, angiogenesis, hemostasis (regulation of blood coagulation), and blood pressure.

The major form of adipose tissue in mammals (commonly referred to as "fat") is white adipose tissue, WAT. It would appear that adipose tissue has the ability to regulate lipid metabolism locally as well as at distant sites such as liver, muscle and brain.

In future, it is likely that the mechanisms that lead to the secondary effects of lipid metabolism on atheroma, immunity and carcinogenesis will be by: lipid [lip´id] a group of substances comprising fatty, greasy, oily, and waxy compounds that are insoluble in water and soluble in nonpolar solvents, such as hexane, ether, and chloroform.

Simple lipids are the triglycerides or neutral fats. Each triglyceride molecule is composed of one molecule of glycerol joined by ester linkages to three fatty acid. Glycerophospholipids, usually referred to as phospholipids (though sphingomyelins are also classified as phospholipids), are ubiquitous in nature and are key components of the lipid bilayer of cells, as well as being involved in metabolism and cell signaling.

Neural tissue (including the brain) contains relatively high amounts of glycerophospholipids, and alterations in their. The series of review articles presented in this book summarizes the recent state of the art in lipid research in a comprehensive way.

The authors provide a general overview of this field and draw the reader’s attention to the most recent : Hardcover. Co-culture of LLC cells promoted a decreased volume of the lipid droplets in 3 T3-L1 cells, compromising its maturation process (adipogenesis) in vitro.

This result was followed by the downregulation of adipogenic and lipolytic gene expression, increased in apoptosis markers and proinflammatory cytokines secretion by both tumor cells and Cited by: 1. The emulsification of dietary fats renders them accessible to various pancreatic lipases in the small intestine.

These lipases, pancreatic lipase and pancreatic phospholipase A 2 (PLA 2) generate free fatty acids and a mixture of mono- and diglycerides from dietary atic lipase degrades triglyceride at the sn-1 and sn-3 positions sequentially.

The book begins by tracing the history of the development of tissue culture. This is followed by separate chapters on early development of cell culture nutrition; the biological effects of serum; the energy metabolism of malignant cells; the gaseous environment of the mammalian cell in culture; and the uptake and utilization of amino acids by Book Edition: 1.

In this issue of Cells, both original research and reviews highlight the new advances being made in the role of lipid droplets in disease. LDs vary in composition by cell type but in mammals they are comprised mostly of the neutral lipids triacylglycerol (TAG) and esterified cholesterol (CE).Author: Paul Dalhaimer.

The important pathways/cycles of lipid metabolism are briefly described: Cycle # 1. Fatty Acid Oxidation: The fatty acids in the body are mostly oxidized by p-oxidation. β-Oxidation may be defined as the oxidation of fatty acids on the p-carbon atom.

This results in the sequential removal of a two carbon fragment, acetyl CoA (Fig. Adipose tissue is now recognized as a widely dispersed secretory organ that exhibits autocrine, paracrine, and endocrine properties, and plays a significant role in obesity, the most common health problem in industrialized countries.

In Adipose Tissue Protocols, Gerard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques. Aging in cell and tissue culture.

New York, Plenum Press, (OCoLC) Online version: Aging in cell and tissue culture. New York, Plenum Press, (OCoLC) Material Type: Conference publication: Document Type: Book: All Authors / Contributors: Ema Holečková; Vincent J Cristofalo; European Tissue Culture Society.

In normal tissue, a portion of the lipid is present as lipid protein complex. This masked fat cannot be demon­strated by histological methods. Treatment with protein-splitting enzymes makes these lipoproteins ‘un­masked’ and can be stained by usual methods.

Such unmasking, called fatty degeneration, also results from anaemia, infection, etc. 2 volumes: 24 cm Includes bibliographical references and index v.

1. Introduction: general pathways in metabolism of lipids in mammalian tissues ; Liver / L.M.G. Van Golde and S.G.

Van den Bergh -- Gastrointestinal tissue / John M. Johnston -- Blood: introductory comments ; Red blood cells / Stephen B. Shohet -- Plasma lipoproteins: structure and metabolism / John P. Pages: @article{osti_, title = {Characterization of lipid metabolism in insulin-sensitive adipocytes differentiated from immortalized human mesenchymal stem cells}, author = {Prawitt, Janne and Niemeier, Andreas and Kassem, Moustapha and Beisiegel, Ulrike and Heeren, Joerg}, abstractNote = {There is a great demand for cell models to study human adipocyte : Prawitt, Janne.X Cholesterol Lipid Concentrate is a protein-free, chemically-defined supplement used for growing NSO and other lipid dependent cell lines to high densities while supporting growth and protein expression without complicating downstream.

Cell and Tissue Culture.